In Vitro Antifungal Activity of Three Synthetic Peptides against Candida auris and Other Candida Species of Medical Importance
نویسندگان
چکیده
Candidiasis is an opportunistic infection affecting immunosuppressed and hospitalized patients, with mortality rates approaching 40% in Colombia. The growing pharmacological resistance of Candida species the emergence multidrug-resistant auris are major public health problems. Therefore, different antimicrobial peptides (AMPs) being investigated as therapeutic alternatives to control candidiasis effectively safely. This work aimed evaluate vitro antifungal activity three synthetic AMPs, PNR20, PNR20-1, 35409, against ATCC reference strains albicans, glabrata, parapsilosis, krusei, tropicalis, clinical isolates C. auris. Antifungal susceptibility testing, determined by broth microdilution, showed that AMPs have planktonic cells all evaluated. In had effect on biofilm formation cell viability, XTT assay flow cytometry, respectively. Also, morphological alterations membrane at intracellular level these were induced peptides, observed transmission electron microscopy. vitro, no cytotoxicity L929 murine fibroblasts. Our results evaluated potential most important Colombia world.
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ژورنال
عنوان ژورنال: Antibiotics
سال: 2023
ISSN: ['2079-6382']
DOI: https://doi.org/10.3390/antibiotics12081234